Exploiting a heterologous construction of the 3-hydroxypropionic acid carbon fixation pathway with mesaconate as an indicator in Saccharomyces cerevisiae

نویسندگان

چکیده

Abstract The 3-Hydroxypropionic acid (3-HP) pathway is one of the six known natural carbon fixation pathways, in which species used bicarbonate. It has been considered to be most suitable for aerobic CO 2 among pathways. Mesaconate a high value-added derivative 3-HP and can as co-monomer produce fire-retardant materials hydrogels. In this study, we use mesaconate reporting compound evaluate construction optimization sub-part Saccharomyces cerevisiae . Combined with fine-tuning malonyl-CoA reductase (MCR-C MCR-N) expression level 3-Hydroxypropionyl-CoA synthase, sub-pathway was optimized using glucose or ethanol substrate, productions reaching 90.78 61.2 mg/L, respectively. Graphical

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Establishing a synthetic pathway for high-level production of 3-hydroxypropionic acid in Saccharomyces cerevisiae via β-alanine.

Microbial fermentation of renewable feedstocks into plastic monomers can decrease our fossil dependence and reduce global CO2 emissions. 3-Hydroxypropionic acid (3HP) is a potential chemical building block for sustainable production of superabsorbent polymers and acrylic plastics. With the objective of developing Saccharomyces cerevisiae as an efficient cell factory for high-level production of...

متن کامل

Engineering and systems-level analysis of Saccharomyces cerevisiae for production of 3-hydroxypropionic acid via malonyl-CoA reductase-dependent pathway

BACKGROUND In the future, oil- and gas-derived polymers may be replaced with bio-based polymers, produced from renewable feedstocks using engineered cell factories. Acrylic acid and acrylic esters with an estimated world annual production of approximately 6 million tons by 2017 can be derived from 3-hydroxypropionic acid (3HP), which can be produced by microbial fermentation. For an economicall...

متن کامل

Production of 3-hydroxypropionic acid from glucose and xylose by metabolically engineered Saccharomyces cerevisiae

Biomass, the most abundant carbon source on the planet, may in the future become the primary feedstock for production of fuels and chemicals, replacing fossil feedstocks. This will, however, require development of cell factories that can convert both C6 and C5 sugars present in lignocellulosic biomass into the products of interest. We engineered Saccharomyces cerevisiae for production of 3-hydr...

متن کامل

Heterologous Production of Dihomo- -Linolenic Acid in Saccharomyces cerevisiae

cerevisiae Saccharomyces-Linolenic Acid in γ Heterologous Production of Dihomo

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

ژورنال

عنوان ژورنال: Bioresources and Bioprocessing

سال: 2023

ISSN: ['2197-4365']

DOI: https://doi.org/10.1186/s40643-023-00652-5