Exploiting a heterologous construction of the 3-hydroxypropionic acid carbon fixation pathway with mesaconate as an indicator in Saccharomyces cerevisiae

Abstract The 3-Hydroxypropionic acid (3-HP) pathway is one of the six known natural carbon fixation pathways, in which species used bicarbonate. It has been considered to be most suitable for aerobic CO 2 among pathways. Mesaconate a high value-added derivative 3-HP and can as co-monomer produce fire-retardant materials hydrogels. In this study, we use mesaconate reporting compound evaluate construction optimization sub-part Saccharomyces cerevisiae . Combined with fine-tuning malonyl-CoA reductase (MCR-C MCR-N) expression level 3-Hydroxypropionyl-CoA synthase, sub-pathway was optimized using glucose or ethanol substrate, productions reaching 90.78 61.2 mg/L, respectively. Graphical